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2-YEAR STUDY IN IPGR “K.MALKOV”, SADOVO

Application in agriculture through the water of information spectral copies (IC) of various biological and chemical substances.

Prof. Tencho Cholakov, PhD
Assoc. Prof. Stanislav Stamatov, PhD
Institute of Plant Genetic Resources “Konstantin Malkov”, Sadovo, Bulgaria.

Beginning and Purpose of the research related to information spectral copies (IS) in IPGR – Sadovo

After the visit in the spring of 2018 to the Institute by Mr. Ognyan Tsanev, manager of AGROBIOVET EOOD, Mr. Atanas Atanasov and Mr. Evgeni Germanov, representatives of “Global IC Technologies” EOOD, we were very surprised by presented information on information copies and water structuring. Therefore, we gladly accepted their invitation for joint scientific and technical cooperation in the field of Plant Breeding.

AIM: The original purpose of the experiments was to obtain information – How do information copies work? Can we successfully use them in our daily activities?

2018 year

In order to realize the goal already in 2018, we launched several experiments:

I. With informative spectral copies containing data for TWO herbicides for peanuts.

II. In the production of pepper seedlings with six spectral copies of biological and chemical substances (with two sowing dates).

 image64  image65

I. Experiments with peanuts

In the IPGR field, two plots sown with peanuts were treated against weeds immediately after sowing with water loaded with informative spectral copies of two different active substances from the “contact” herbicide group. A control sample variant, treated with clean water, was also placed. The results of the experimental plots were recorded between the 25th and 30th day after the germination of the culture.

Results:

It was found that in both variants treated with IC of soil herbicides, the effect of IC application was about 95% (there were single emerged weeds), and in the control sample, weeding was significant:

 image66  image67 image68

II. Experiments with pepper

А). 1st date
On a special site on July 24 (outdoors, due to the high air temperatures), an experiment with 6 variants and a control sample was set. The pepper seeds were sown in a peat mixture (3:1) in plastic terrines with 45 nests each. For each variant, 4 terrines were used, which were well watered after sowing. For the study, 6 disks containing information copies (the spectral characteristics of the substances) were used, respectively, of:
1. Atonic
2. Indole butyric acid
3. Neemoil
4. Triacontanol
5. Humic acids
6. CCC (chloro choline chloride)
7. Control sample (water without IC)

After seeding, a plastic bottle (1.5 L) of water was placed on top of each disc to “charge” for 1–2 h. Then, with this water containing informational spectral copies, the corresponding one of the variants (terrines with pepper seeds) was poured. For a longer impact of the information copies, each of the carriers (CD) of the spectral information was laid in the middle under the terrines of each variant. A distance was provided between the individual variants to avoid mutual influence of the IC.

The seeds germinated normally in all variants. Germination began on July 31, and the mass emergence phase was recorded on August 3. Due to the high air temperatures, the mass emergence occurred at the same time, so at this stage it is difficult to indicate which of the options is the best. Subsequently, due to the very high temperatures, a “false” cutting of seedlings appeared in the variants, and this experiment was not carried out to the end. Therefore, during the following observations, we only made findings, but it was observed that the seedlings in the terrines were in the best condition and development, treated with water containing IC of the substances Indole butyric acid, Neemoil and CCC (chlorine choline chloride).

var.2

var.2

Контрола

Control sample

var.5

var.5

var.4

var.4

In the 5th variant, the number of plants that failed after germination was the highest (35-45%).

B). 2nd date

An experiment similar to the one described above with pepper, but in a steel-glass vegetation house, was started on September 5, 2018. The seeds were sown in terrines on concrete surfaces on September 5. For pepper with a second sowing date, 6 discs containing informative spectral copies were also used. The arrangement of the variants and their treatment with “charged” water proceeded in a manner identical to the first experiment.

 image73  image74

From Table 1, it can be seen that on the second date of pepper sowing, the beginning of seed germination was registered on 14.09. only in the 2nd and 4th variants. However, mass sprouting of plants was reported on 16.09. only on the third variant (IC of Neemoil), and one day later on all other variants treated with IC.

Result:

The differences between the variants when using IS can be seen from the results placed in Table 1. The differences compared to the control sample in this experiment reach up to 5 days.

Table 1

№ Variants with IC of substances: Phenological development in pepper
Phase Emergence (% emerged from the number of all nests) Phase One Real Sheet (ORS)
% of emerged on 14.09 % of emerged on 16.09 % of emerged 17.09 en masse* % of plants entering ORS phase on date:
24.09 26.09 27.09 28.09 01.10
1. Atonic – 12,2 53,3 5,4 22,2 40,2 48,8 53,2
2. Indole butyric acid 1,7 40,0 88,9 13,8 55,5* 75,6 82,2 95,4
3. Neemoil – 51,1* 94,0 15,5 62,2* 66,7 77,7 95,4
4. Triacontanol 1,1 31,6 85,5 12,2 48,8 62,0 71,1 77,8
5. Humic acids  – 44,4 92,2 11,0 41,6 60,0 60,1 73,3
6. ССС – 35,6 91,1 15,6 62,2* 64,4 75,7 93,0
7. Control sample(Water without IC) – 28,2 45,5* 7,8 22,0 31,1 46,5 51,0*
* Mass occurrence of the phase – the signs characteristic of the given phase are observed in more than 50% of the examined plants!

Conclusions – 2018 year (The data are for one year!!)

– The results of the water application of information spectral copies of soil herbicides in field conditions in peanuts show a positive trend for the influence of IC on emerging weeds and a certain effect that can be realized with their inclusion as an element of the technology.

– In the case of pepper, research is in the initial phases (seedling production), but here too a positive effect on the speed of development is observed with the use of informative spectral copies of Indolebutyric acid, Neemoil and CCC.

– Agricultural production is impossible without water and this reveals a huge potential for research and inclusion of water containing informative spectral copies of various biological and chemical substances in every production technology.

2019 year

In 2019, Information Spectral Copies (IC) experiments continue:

I. For peanuts in field conditions: Testing different concentration of 4 soil herbicides (Table 2).

Table 2 

Variants with tested ICs of 4 herbicides with different degrees of dilution (concentration):

 Variants preparation
1 Trifluralin
 2 Trifluralin
 3 Metolachlor
 4 Metolachlor
 5 Pendimethalin
 6 Pendimethalin
 7 Imazethapyr
 8 Imazethapyr
 Control sample Clear water

After treatment with IC herbicides in the row there is no weeding in contrast to the control sample:

image24
The control sample:

 image25 image26

II. Experiments with pepper.

A) Kurtovska capia pepper seedlings were produced in a water bed in a steel-glass vegetation house and planted in the field with the aim of preliminary study of the effect of IC application of complex mineral fertilizers on the biological manifestations of pepper in field conditions.

For the field trials with fertilizer IC testing:
• For fertilizing the pepper, 500 liters/ha of vermicompost (organic fertilizer from California worms) was used before planting, as the main, background fertilization
• As vegetation nutrients, complex fertilizers NPK 10-45-10, NPK 15-10-30 and Calcium nitrate Ca(NO3)2 introduced by fertigation according to the scheme. Watering of part of the crop with “structured” water containing IC of the above-mentioned fertilizers is carried out.

B) Experiments in a vegetation house with IC of substances.

image33_1

Table 3

Results of a study of the influence of IC on the occurrence of some phenophases in pepper grown in a water bed in 2019 (1st date):

№ Variants with IC of substances:  Phenological phases
 Date / Emerged
(% from all plants)
 Date / Phase first
real sheet (crossover)
(% from all plants)
 Date / Fourth
real sheet (% from all plants)
 22.05 23.05  24.05 25.05  29.0
5
 31.0
5
01.0
6
 02.0
6
 03.0
6
 05.0
6
07.0
6
 09.0
6
 1 Indole butyric acid  19.0 66.4 – 28.6 50.4 – – 6.7 12.4 50.4 –
 2 Neemoil  6.7 47.6 62.8 – 32.4 52.3 – – 3.8 9.5 39.0  60.1
 3 Control sample  6.7  51.4 58.0  – 10.5 37.1 49.5 60.9  – 5.7 37.1  63.8
 4 Streptomycin  6.7 23.8 44.7 64.7  13.3 39.0 47.5 58.0 – 0.9 14.3  56.2 
 5 ССС  5.6 22.8 45.7 61.9 1.9 15.2 34.3 50.5 – 3.8 31.4  59.0 

image36

Table 4

Biometric indicators of 30-day-old seedlings (pepper) using IC of various biological and chemical substances:

Variants with IC of substances: Above ground
fresh
biomass

(g)
Number of
leaves
 Leaves
weight
(g)
 Stem
height
(cm)
Stem
diameter
(cm)
 1. Indole butyric
acid
7.15* 7.41* 3.52* 31,7 3.37*
 2. Neemoil 5.71 6.67 2.51 31.9 3.12
 3. Control sample
(without IC)
5.21 6.50 2.34 30.3 2.91
 4. Streptomycin 2.27* 4.75* 1.13* 17.8* 2.80
 5. ССС 4.53 6.17 1.98 28.7 3.04
 * – LSD 0,05%

Conclusion

  • For the second year in the field for peanuts, the results in the IC-treated variants of soil herbicides were encouraging and indicated that there was a significant effect of the IC application. For now, there are still additional questions related to optimizing the dose of herbicides applied through IC, but research continues.
  • In experiments with the cultivation of pepper seedlings in a water bed using IC of different substances (biological and chemical), it was found that the seedlings of the variant with Indole butyric acid had better agrotechnical indicators (fresh biomass, number and mass of leaves and stem diameter) relative to the control sample. The differences are statistically proven. In this variant, the trend established in 2018 for faster development of young plants was also confirmed, but this year, due to the higher temperature background, the differences are up to 2 days.
  • In Polish conditions, during the experiments with pepper, our expectations are to get answers to a number of questions, incl. and for the possibility of conducting future research to develop biomanufacturing technology.

image34_1

We express our great gratitude to the sponsor of the scientific research company “Agrobiovet” EOOD!!!

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