Prof. Tencho Cholakov, PhD
Assoc. Prof. Stanislav Stamatov, PhD
Institute of Plant Genetic Resources “Konstantin Malkov”, Sadovo, Bulgaria.
After the visit in the spring of 2018 to the Institute by Mr. Ognyan Tsanev, manager of AGROBIOVET EOOD, Mr. Atanas Atanasov and Mr. Evgeni Germanov, representatives of “Global IC Technologies” EOOD, we were very surprised by presented information on information copies and water structuring. Therefore, we gladly accepted their invitation for joint scientific and technical cooperation in the field of Plant Breeding.
AIM: The original purpose of the experiments was to obtain information – How do information copies work? Can we successfully use them in our daily activities?
In order to realize the goal already in 2018, we launched several experiments:
I. With informative spectral copies containing data for TWO herbicides for peanuts.
II. In the production of pepper seedlings with six spectral copies of biological and chemical substances (with two sowing dates).
In the IPGR field, two plots sown with peanuts were treated against weeds immediately after sowing with water loaded with informative spectral copies of two different active substances from the “contact” herbicide group. A control sample variant, treated with clean water, was also placed. The results of the experimental plots were recorded between the 25th and 30th day after the germination of the culture.
Results:
It was found that in both variants treated with IC of soil herbicides, the effect of IC application was about 95% (there were single emerged weeds), and in the control sample, weeding was significant:
II. Experiments with pepper
А). 1st date
On a special site on July 24 (outdoors, due to the high air temperatures), an experiment with 6 variants and a control sample was set. The pepper seeds were sown in a peat mixture (3:1) in plastic terrines with 45 nests each. For each variant, 4 terrines were used, which were well watered after sowing. For the study, 6 disks containing information copies (the spectral characteristics of the substances) were used, respectively, of:
1. Atonic
2. Indole butyric acid
3. Neemoil
4. Triacontanol
5. Humic acids
6. CCC (chloro choline chloride)
7. Control sample (water without IC)
After seeding, a plastic bottle (1.5 L) of water was placed on top of each disc to “charge” for 1–2 h. Then, with this water containing informational spectral copies, the corresponding one of the variants (terrines with pepper seeds) was poured. For a longer impact of the information copies, each of the carriers (CD) of the spectral information was laid in the middle under the terrines of each variant. A distance was provided between the individual variants to avoid mutual influence of the IC.
The seeds germinated normally in all variants. Germination began on July 31, and the mass emergence phase was recorded on August 3. Due to the high air temperatures, the mass emergence occurred at the same time, so at this stage it is difficult to indicate which of the options is the best. Subsequently, due to the very high temperatures, a “false” cutting of seedlings appeared in the variants, and this experiment was not carried out to the end. Therefore, during the following observations, we only made findings, but it was observed that the seedlings in the terrines were in the best condition and development, treated with water containing IC of the substances Indole butyric acid, Neemoil and CCC (chlorine choline chloride).
In the 5th variant, the number of plants that failed after germination was the highest (35-45%).
B). 2nd date
An experiment similar to the one described above with pepper, but in a steel-glass vegetation house, was started on September 5, 2018. The seeds were sown in terrines on concrete surfaces on September 5. For pepper with a second sowing date, 6 discs containing informative spectral copies were also used. The arrangement of the variants and their treatment with “charged” water proceeded in a manner identical to the first experiment.
From Table 1, it can be seen that on the second date of pepper sowing, the beginning of seed germination was registered on 14.09. only in the 2nd and 4th variants. However, mass sprouting of plants was reported on 16.09. only on the third variant (IC of Neemoil), and one day later on all other variants treated with IC.
Result:
The differences between the variants when using IS can be seen from the results placed in Table 1. The differences compared to the control sample in this experiment reach up to 5 days.
Table 1
№ | Variants with IC of substances: | Phenological development in pepper | |||||||
---|---|---|---|---|---|---|---|---|---|
Phase Emergence (% emerged from the number of all nests) | Phase One Real Sheet (ORS) | ||||||||
% of emerged on 14.09 | % of emerged on 16.09 | % of emerged 17.09 en masse* | % of plants entering ORS phase on date: | ||||||
24.09 | 26.09 | 27.09 | 28.09 | 01.10 | |||||
1. | Atonic | – | 12,2 | 53,3 | 5,4 | 22,2 | 40,2 | 48,8 | 53,2 |
2. | Indole butyric acid | 1,7 | 40,0 | 88,9 | 13,8 | 55,5* | 75,6 | 82,2 | 95,4 |
3. | Neemoil | – | 51,1* | 94,0 | 15,5 | 62,2* | 66,7 | 77,7 | 95,4 |
4. | Triacontanol | 1,1 | 31,6 | 85,5 | 12,2 | 48,8 | 62,0 | 71,1 | 77,8 |
5. | Humic acids | – | 44,4 | 92,2 | 11,0 | 41,6 | 60,0 | 60,1 | 73,3 |
6. | ССС | – | 35,6 | 91,1 | 15,6 | 62,2* | 64,4 | 75,7 | 93,0 |
7. | Control sample(Water without IC) | – | 28,2 | 45,5* | 7,8 | 22,0 | 31,1 | 46,5 | 51,0* |
* Mass occurrence of the phase – the signs characteristic of the given phase are observed in more than 50% of the examined plants! |
– The results of the water application of information spectral copies of soil herbicides in field conditions in peanuts show a positive trend for the influence of IC on emerging weeds and a certain effect that can be realized with their inclusion as an element of the technology.
– In the case of pepper, research is in the initial phases (seedling production), but here too a positive effect on the speed of development is observed with the use of informative spectral copies of Indolebutyric acid, Neemoil and CCC.
– Agricultural production is impossible without water and this reveals a huge potential for research and inclusion of water containing informative spectral copies of various biological and chemical substances in every production technology.
In 2019, Information Spectral Copies (IC) experiments continue:
I. For peanuts in field conditions: Testing different concentration of 4 soil herbicides (Table 2).
Table 2
Variants with tested ICs of 4 herbicides with different degrees of dilution (concentration):
Variants | preparation |
1 | Trifluralin |
2 | Trifluralin |
3 | Metolachlor |
4 | Metolachlor |
5 | Pendimethalin |
6 | Pendimethalin |
7 | Imazethapyr |
8 | Imazethapyr |
Control sample | Clear water |
After treatment with IC herbicides in the row there is no weeding in contrast to the control sample:
II. Experiments with pepper.
A) Kurtovska capia pepper seedlings were produced in a water bed in a steel-glass vegetation house and planted in the field with the aim of preliminary study of the effect of IC application of complex mineral fertilizers on the biological manifestations of pepper in field conditions.
For the field trials with fertilizer IC testing:
• For fertilizing the pepper, 500 liters/ha of vermicompost (organic fertilizer from California worms) was used before planting, as the main, background fertilization
• As vegetation nutrients, complex fertilizers NPK 10-45-10, NPK 15-10-30 and Calcium nitrate Ca(NO3)2 introduced by fertigation according to the scheme. Watering of part of the crop with “structured” water containing IC of the above-mentioned fertilizers is carried out.
Table 3
Results of a study of the influence of IC on the occurrence of some phenophases in pepper grown in a water bed in 2019 (1st date):
№ | Variants with IC of substances: | Phenological phases | |||||||||||
Date / Emerged (% from all plants) |
Date / Phase first real sheet (crossover) (% from all plants) |
Date / Fourth real sheet (% from all plants) |
|||||||||||
22.05 | 23.05 | 24.05 | 25.05 | 29.0 5 |
31.0 5 |
01.0 6 |
02.0 6 |
03.0 6 |
05.0 6 |
07.0 6 |
09.0 6 |
||
1 | Indole butyric acid | 19.0 | 66.4 | – | 28.6 | 50.4 | – | – | 6.7 | 12.4 | 50.4 | – | |
2 | Neemoil | 6.7 | 47.6 | 62.8 | – | 32.4 | 52.3 | – | – | 3.8 | 9.5 | 39.0 | 60.1 |
3 | Control sample | 6.7 | 51.4 | 58.0 | – | 10.5 | 37.1 | 49.5 | 60.9 | – | 5.7 | 37.1 | 63.8 |
4 | Streptomycin | 6.7 | 23.8 | 44.7 | 64.7 | 13.3 | 39.0 | 47.5 | 58.0 | – | 0.9 | 14.3 | 56.2 |
5 | ССС | 5.6 | 22.8 | 45.7 | 61.9 | 1.9 | 15.2 | 34.3 | 50.5 | – | 3.8 | 31.4 | 59.0 |
Table 4
Biometric indicators of 30-day-old seedlings (pepper) using IC of various biological and chemical substances:
Variants with IC of substances: | Above ground fresh biomass (g) |
Number of leaves |
Leaves weight (g) |
Stem height (cm) |
Stem diameter (cm) |
1. Indole butyric acid |
7.15* | 7.41* | 3.52* | 31,7 | 3.37* |
2. Neemoil | 5.71 | 6.67 | 2.51 | 31.9 | 3.12 |
3. Control sample (without IC) |
5.21 | 6.50 | 2.34 | 30.3 | 2.91 |
4. Streptomycin | 2.27* | 4.75* | 1.13* | 17.8* | 2.80 |
5. ССС | 4.53 | 6.17 | 1.98 | 28.7 | 3.04 |
* – LSD 0,05% |